https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51311 Wed 28 Feb 2024 10:53:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47219 Wed 28 Feb 2024 10:12:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23171 Wed 24 Aug 2016 16:12:39 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31947 2 emissions from the transport sector will increase the risks borne by society as a result of future global uncertainty, the uncertain timing of globally coordinated action on climate change and the timing of peak oil. We also find that the success of local policy is affected by the global future which prevails. The use of the Ecological Footprint allows policy to be informed by the consequences of both CO₂ emissions and increasing demand for land. The study provides a decision-making framework that allows local decision makers to make robust policy despite global uncertainty. This framework has wider applicability to other nations and/or subnational jurisdictions worldwide.]]> Wed 20 Jan 2021 17:16:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:12527 Wed 11 Apr 2018 16:10:30 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42841 Wed 07 Sep 2022 14:48:05 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:52517 Wed 06 Mar 2024 15:33:03 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8558 Thu 30 Jan 2014 14:54:51 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8544 Thu 30 Jan 2014 14:42:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8549 Thu 30 Jan 2014 14:42:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8550 Thu 30 Jan 2014 14:39:42 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:8555 Thu 30 Jan 2014 14:36:56 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:37182 Acinetobacter baumannii has rapidly emerged as a major cause of gram-negative hospital infections worldwide. A. baumannii encodes for the transport protein AceI, which confers resistance to chlorhexidine, a widely used antiseptic. AceI is also the prototype for the recently discovered proteobacterial antimicrobial compound efflux (PACE) family of transport proteins that confer resistance to a range of antibiotics and antiseptics in many gram-negative bacteria, including pathogens. The gene encoding AceI is conserved in the core genome of A. baumannii, suggesting that it has an important primordial function. This is incongruous with the sole characterized substrate of AceI, chlorhexidine, an entirely synthetic biocide produced only during the last century. Here we investigated a potential primordial function of AceI and other members of the PACE family in the transport of naturally occurring polyamines. Polyamines are abundant in living cells, where they have physiologically important functions and play multifaceted roles in bacterial infection. Gene expression studies revealed that the aceI gene is induced in A. baumannii by the short-chain diamines cadaverine and putrescine. Membrane transport experiments conducted in whole cells of A. baumannii and Escherichia coli and also in proteoliposomes showed that AceI mediates the efflux of these short-chain diamines when energized by an electrochemical gradient. Assays conducted using 8 additional diverse PACE family proteins identified 3 that also catalyze cadaverine transport. Taken together, these results demonstrate that short-chain diamines are common substrates for the PACE family of transport proteins, adding to their broad significance as a novel family of efflux pumps.]]> Thu 27 Aug 2020 12:47:49 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:563 Thu 25 Jul 2013 09:10:40 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:508 Thu 25 Jul 2013 09:10:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:3 Thu 25 Jul 2013 09:10:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:11 Thu 25 Jul 2013 09:10:12 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43411 Thu 20 Oct 2022 09:55:52 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48842 Thu 13 Apr 2023 09:46:01 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:1434 Sat 24 Mar 2018 08:28:04 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:10425 Sat 24 Mar 2018 08:13:15 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21312 Sat 24 Mar 2018 07:52:46 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5154 Sat 24 Mar 2018 07:49:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5067 Sat 24 Mar 2018 07:48:47 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:5332 Sat 24 Mar 2018 07:45:54 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:130 Sat 24 Mar 2018 07:43:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:22 Sat 24 Mar 2018 07:42:07 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51 Sat 24 Mar 2018 07:42:01 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:25273 Sat 24 Mar 2018 07:38:17 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27633 L-tryptophan > L-tyrosine) were substrates for this enzyme, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death. This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a majority of LAAO activity to the sperm head. Equine cryopreservation medium was shown to contain sufficient quantities of aromatic amino acids to activate the LAAO system and generate ROS. The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the cryopreservation of equine spermatozoa. It is possible that inhibitors of this enzyme system may facilitate the development of modified cryostorage regimes for clinical validation in vivo.]]> Sat 24 Mar 2018 07:34:05 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:23693 Sat 24 Mar 2018 07:13:26 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38651 Mon 29 Jan 2024 17:49:32 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:31302 Mon 23 Sep 2019 10:27:16 AEST ]]>